Embryotoxicity estimation of commonly used compounds with embryonic stem cell test

Embryotoxicity estimation of commonly used compounds with embryonic stem cell test

The embryonic stem cell test (EST), an alternative model to animal studies, is a reliable and scientifically validated in vitro system for testing embryotoxicity. In contrast to most in vivo animal tests, two permanent cell lines, murine fibroblasts (BALB/c‑3T3 cells) and murine embryonic stem cells (mES‑D3 cells), are used in EST instead of animals in standard tests of toxicity. The embryotoxi...

Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test.

Rhizoma Atractylodes macrocephala, Radix Isatidis, Coptis chinensis and Flos Genkwa are common herbal remedies used by pregnant woman in China. In this study, their potential embryotoxicity was assessed using the embryonic stem cell test (EST) and a prediction model. The potential embryotoxicity of the herbs was based on three endpoints: the concentrations of the compounds that inhibited the pr...

Assessment of Embryotoxicity and Teratogenicity by the Embryonic Stem Cell Test

During pregnancy, a fetus can be affected by exposure to a variety of chemicals and pharmaceuticals. These effects can occur through exposure of the mother and subsequent placental transport. Thus, it is important to assess embryotoxicity (developmental toxicity without the observation of maternal toxicity) and teratogenicity (irreversible structure developmental effects without the observation...

Differentiation of Mouse Embryonic Stem Cell into Insulin-Secreting Cell

Purpose: Differentiation of mouse embryonic stem cells into Insulin secreting endocrine cells. Materials and Methods: In this study, Royan B1 mouse embryonic stem cell (derived from C57BL/6 mouse) were used. In directed differentiation method, embryonicstem cells after embryoid bodies formation were differentiated into insulin secreting cells. Nestin positive cells were obtained after culture ...

Reevaluation of the embryonic stem cell test